The polyclonal cell line K562-Luc/mCherry is a derivative of the wildtype K562 lymphoblast cell line achieved through transduction with a lentiviral expression vector. This vector encodes the firefly luciferase gene (luc2) controlled by the EF-1 alpha promoter, along with the genes for Puromycin N-acetyl-transferase and mCherry, linked by T2A cleavage peptide, and regulated by the CMV promoter. K562-Luc/mCherry demonstrates stable expression of luciferase, resistance to Puromycin, and can be easily visualized via red fluorescence (Ex 587nm, Em 610nm).