The polyclonal cell line K562-Luc is a derivative of the wildtype K562 lymphoblast cell line achieved through transduction with a lentiviral expression vector. This vector encodes the codon-optimized firefly luciferase gene (luc2) controlled by the EF-1 alpha promoter, along with the gene for Puromycin N-acetyl-transferase regulated by the hPGK promoter. K562-Luc demonstrates stable expression of luciferase and resistance to Puromycin.
K562-Luc is well suited for quantitative cell viability and proliferation assays as well as cell-based cytotoxicity and immune effector assays, where luciferase signal directly correlates with viable target cell number. In addition, the stable EF-1α-driven luc2 expression enables sensitive bioluminescence-based in vivo imaging for longitudinal monitoring of tumor burden and treatment response.
Key Features
- Firefly luciferase reporter activity enabling straightforward use in in-vitro cytotoxicity assays and in-vivo bioluminescence imaging.
- Stable expression has been verified for 15 passages.
- The cell line has been verified to be mycoplasma-free, and cell line authentication has been confirmed.
Applications
- Immune effector cell assays
- T cell or NK cell dependent cytotoxicity (TDCC, NKDCC)
- Antibody dependent cytotoxicity (ADCC)
- CAR-T / CAR-NK assays
- Bispecific antibody testing
- Cell viability & proliferation assays
- Drug cytotoxicity
- Growth inhibition
- Long-term proliferation
- In-vivo Imaging
- Tumor growth monitoring
- Metastasis studies
- Therapy response over time
Shipping & Storage
- Content: 1 cryovial containing 5 Mio cells in 1ml freezing medium (RPMI1640 with 10% FBS and 5% DMSO)
- Shipping: On dry ice
- storage: Liquid nitrogen vapor phase
Important:
Store cell cryovials in liquid nitrogen immediately or start culture according to product sheet instructions. Never store cell vials at -80°C as cell viability will decrease.
Cell lines are supplied for research use only and are subject to a Limited Use License as described in the Terms & Conditions. Additional usage rights may be available upon request.