Cell culturing is the growth of cells outside of the prenatal organism under controlled environmental conditions and is an essential part in biomedical research and biotechnology. The constant and professional maintenance of cells according to specific culture conditions for each cell type is directly connected to the success of experimental research and biomolecule production.
Read More
Cell viability and cytotoxicity assays are powerful tools investigating the effect of various treatments (e.g. chemical, environmental) for their effect on cell growth and viability.
In vitro proliferation assays determine the number of viable cells and are based on quantifying the number of cell nuclei, metabolic activity, or mitochondrial integrity. Together with cytotoxicity and apoptosis assays, they help assess the cellular response to chemical compounds, novel therapeutics, or environmental factors.
Additionally, cell-dependent cytotoxicity assays evaluate immune-mediated target cell destruction. These include T-cell-dependent cytotoxicity assays (TDCC), natural killer (NK) cell-mediated cytotoxicity assays (NKCC), and antibody-dependent cell-mediated cytotoxicity (ADCC) assays, which analyze the role of antibodies in mediating immune responses against target cells.